| Version |
2.0.1.0 |
| Include contaminants |
TRUE |
| PSM FDR |
0.01 |
| PSM FDR Crosslink |
0.01 |
| Protein FDR |
0.01 |
| Site FDR |
0.01 |
| Use Normalized Ratios For Occupancy |
TRUE |
| Min. peptide Length |
7 |
| Min. score for unmodified peptides |
0 |
| Min. score for modified peptides |
40 |
| Min. delta score for unmodified peptides |
0 |
| Min. delta score for modified peptides |
6 |
| Min. unique peptides |
0 |
| Min. razor peptides |
1 |
| Min. peptides |
1 |
| Fixed modification |
|
| Use only unmodified peptides and |
TRUE |
| Modifications included in protein quantification |
Oxidation (M);Acetyl (Protein N-term) |
| Peptides used for protein quantification |
Razor |
| Discard unmodified counterpart peptides |
TRUE |
| Label min. ratio count |
2 |
| Use delta score |
FALSE |
| iBAQ |
FALSE |
| iBAQ log fit |
FALSE |
| Match between runs |
TRUE |
| Matching time window [min] |
0.7 |
| Match ion mobility window [indices] |
0.05 |
| Alignment time window [min] |
20 |
| Alignment ion mobility window [indices] |
1 |
| Find dependent peptides |
TRUE |
| Dependent peptide FDR |
0.01 |
| Mass bin size |
0.0065 |
| Decoy mode |
revert |
| Include contaminants |
TRUE |
| Advanced ratios |
TRUE |
| Second peptides |
TRUE |
| Stabilize large LFQ ratios |
TRUE |
| Separate LFQ in parameter groups |
FALSE |
| Require MS/MS for LFQ comparisons |
TRUE |
| Calculate peak properties |
FALSE |
| Main search max. combinations |
200 |
| Advanced site intensities |
TRUE |
| Write msScans table |
FALSE |
| Write msmsScans table |
TRUE |
| Write ms3Scans table |
TRUE |
| Write allPeptides table |
TRUE |
| Write mzRange table |
TRUE |
| Write DIA fragments table |
FALSE |
| Write DIA fragments quant table |
FALSE |
| Write pasefMsmsScans table |
TRUE |
| Write accumulatedMsmsScans table |
TRUE |
| Max. peptide mass [Da] |
4600 |
| Min. peptide length for unspecific search |
8 |
| Max. peptide length for unspecific search |
25 |
| Razor protein FDR |
TRUE |
| Disable MD5 |
FALSE |
| Max mods in site table |
3 |
| Match unidentified features |
FALSE |
| Evaluate variant peptides separately |
TRUE |
| Variation mode |
None |
| MS/MS tol. (FTMS) |
20 ppm |
| Top MS/MS peaks per Da interval. (FTMS) |
12 |
| Da interval. (FTMS) |
100 |
| MS/MS deisotoping (FTMS) |
TRUE |
| MS/MS deisotoping tolerance (FTMS) |
7 |
| MS/MS deisotoping tolerance unit (FTMS) |
ppm |
| MS/MS higher charges (FTMS) |
TRUE |
| MS/MS water loss (FTMS) |
TRUE |
| MS/MS ammonia loss (FTMS) |
TRUE |
| MS/MS dependent losses (FTMS) |
TRUE |
| MS/MS recalibration (FTMS) |
FALSE |
| MS/MS tol. (Unknown) |
20 ppm |
| Top MS/MS peaks per Da interval. (Unknown) |
12 |
| Da interval. (Unknown) |
100 |
| MS/MS deisotoping (Unknown) |
TRUE |
| MS/MS deisotoping tolerance (Unknown) |
7 |
| MS/MS deisotoping tolerance unit (Unknown) |
ppm |
| MS/MS higher charges (Unknown) |
TRUE |
| MS/MS water loss (Unknown) |
TRUE |
| MS/MS ammonia loss (Unknown) |
TRUE |
| MS/MS dependent losses (Unknown) |
TRUE |
| MS/MS recalibration (Unknown) |
FALSE |
| Site tables |
Deamidation (NQ)Sites.txt;Oxidation (M)Sites.txt;Phospho (STY)Sites.txt |
| Enzyme |
Trypsin/P |
| Enzyme mode |
Specific |
| Use enzyme first search |
FALSE |
| Use variable modifications first search |
FALSE |
| Requantify |
TRUE |
| Multiplicity |
2 |
| Max. missed cleavages |
2 |
| Max. labeled AAs |
3 |
| Labels0 |
|
| Labels1 |
Arg10;Lys8 |
| LC-MS run type |
Standard |