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. 2023 Feb 10;4(1):102104. doi: 10.1016/j.xpro.2023.102104
Parameter Value
Version 2.0.1.0
Include contaminants TRUE
PSM FDR 0.01
PSM FDR Crosslink 0.01
Protein FDR 0.01
Site FDR 0.01
Use Normalized Ratios For Occupancy TRUE
Min. peptide Length 7
Min. score for unmodified peptides 0
Min. score for modified peptides 40
Min. delta score for unmodified peptides 0
Min. delta score for modified peptides 6
Min. unique peptides 0
Min. razor peptides 1
Min. peptides 1
Fixed modification
Use only unmodified peptides and TRUE
Modifications included in protein quantification Oxidation (M);Acetyl (Protein N-term)
Peptides used for protein quantification Razor
Discard unmodified counterpart peptides TRUE
Label min. ratio count 2
Use delta score FALSE
iBAQ FALSE
iBAQ log fit FALSE
Match between runs TRUE
Matching time window [min] 0.7
Match ion mobility window [indices] 0.05
Alignment time window [min] 20
Alignment ion mobility window [indices] 1
Find dependent peptides TRUE
Dependent peptide FDR 0.01
Mass bin size 0.0065
Decoy mode revert
Include contaminants TRUE
Advanced ratios TRUE
Second peptides TRUE
Stabilize large LFQ ratios TRUE
Separate LFQ in parameter groups FALSE
Require MS/MS for LFQ comparisons TRUE
Calculate peak properties FALSE
Main search max. combinations 200
Advanced site intensities TRUE
Write msScans table FALSE
Write msmsScans table TRUE
Write ms3Scans table TRUE
Write allPeptides table TRUE
Write mzRange table TRUE
Write DIA fragments table FALSE
Write DIA fragments quant table FALSE
Write pasefMsmsScans table TRUE
Write accumulatedMsmsScans table TRUE
Max. peptide mass [Da] 4600
Min. peptide length for unspecific search 8
Max. peptide length for unspecific search 25
Razor protein FDR TRUE
Disable MD5 FALSE
Max mods in site table 3
Match unidentified features FALSE
Evaluate variant peptides separately TRUE
Variation mode None
MS/MS tol. (FTMS) 20 ppm
Top MS/MS peaks per Da interval. (FTMS) 12
Da interval. (FTMS) 100
MS/MS deisotoping (FTMS) TRUE
MS/MS deisotoping tolerance (FTMS) 7
MS/MS deisotoping tolerance unit (FTMS) ppm
MS/MS higher charges (FTMS) TRUE
MS/MS water loss (FTMS) TRUE
MS/MS ammonia loss (FTMS) TRUE
MS/MS dependent losses (FTMS) TRUE
MS/MS recalibration (FTMS) FALSE
MS/MS tol. (Unknown) 20 ppm
Top MS/MS peaks per Da interval. (Unknown) 12
Da interval. (Unknown) 100
MS/MS deisotoping (Unknown) TRUE
MS/MS deisotoping tolerance (Unknown) 7
MS/MS deisotoping tolerance unit (Unknown) ppm
MS/MS higher charges (Unknown) TRUE
MS/MS water loss (Unknown) TRUE
MS/MS ammonia loss (Unknown) TRUE
MS/MS dependent losses (Unknown) TRUE
MS/MS recalibration (Unknown) FALSE
Site tables Deamidation (NQ)Sites.txt;Oxidation (M)Sites.txt;Phospho (STY)Sites.txt
Enzyme Trypsin/P
Enzyme mode Specific
Use enzyme first search FALSE
Use variable modifications first search FALSE
Requantify TRUE
Multiplicity 2
Max. missed cleavages 2
Max. labeled AAs 3
Labels0
Labels1 Arg10;Lys8
LC-MS run type Standard