REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PE/Cyanine7 anti-human CD56 (NCAM) antibody	BioLegend	Cat# 318318, RRID: AB_604107
Chemicals, peptides, and recombinant proteins
H2O, LC-MS grade	Supelco	7732-18-5
Precision Plus Protein™ Dual Color Standards	Bio-Rad	1610374
10× Tris/Glycine/SDS	Bio-Rad	1610732
2× Laemmli sample buffer	Bio-Rad	1610737
InstantBlue	Abcam	ISB1L-1L
Dithiothreitol (DTT)	Fisher Scientific	3483-12-3
2-Cloroacetamide (CAA)	Aldrich	22790-250G-F
Trypsin	Promega	V5111
Formic acid LC-MS grade	ThermoScientific	85178
Ammonium bicarbonate	Fluka	09830-500G
Acetonitrile	Supelco	EM-AX0145P-6
Urea	Sigma-Aldrich	U5128
Tris-HCl	Merck	1185-53-1
NaCl	Merck	7647-14-5
EDTA	Merck	60-00-4
16% formaldehyde, methanol-free (PFA)	Thermo Fisher Scientific	Cat #28908
Methanol HPLC grade	Sigma-Aldrich	900688
RPMI 1640 Medium for SILAC	Thermo Fisher Scientific	Cat# 88365
DMEM:F-12 for SILAC	Thermo Fisher Scientific	Cat# 88370
Fetal bovine serum	Sigma-Aldrich	Cat# F0392
L-Arginine monohydrochloride	Sigma-Aldrich	Cat# A6969
L-Arginine · HCl (13C6, 99%; 15N4, 99%)	Cambridge Isotope Laboratories	Cat# CNLM-539-H-0.05
L-Lysine monohydrochloride	Sigma-Aldrich	Cat# L8662
L-Lysine · 2HCl (13C6, 99%; 15N2, 99%)	Cambridge Isotope Laboratories	Cat# CNLM-291-H-0.05
L-Leucine	Sigma-Aldrich	Cat# L8912
Penicillin/Streptomycin	Lonza	Cat# DE17-602E
Puromycin dihydrochloride	Santa Cruz	Cat# CAS 58-58-2
Dulbecco’s phosphate-buffered saline (DPBS), 1×, no calcium, no magnesium	Thermo Fisher Scientific	Cat# 10010015
AutoMACS Running Buffer	Miltenyi Biotec	Cat #130-091-221
Dulbecco’s phosphate-buffered saline (DPBS), 1× with Ca2+ & Mg2+	Thermo Fisher Scientific	Cat# 14040-091
PBS with Mg2+ and Ca2+ (PBS/Mg/Ca)	Thermo Fisher Scientific	Cat# 14040133
Trypsin-EDTA	Thermo Fisher Scientific	Cat# 25300062
Critical commercial assays
MycoAlert Mycoplasma Detection kit	Lonza	Cat# LT07-318
Live-or-Dye NucFix Red Staining kit	Biotium	Cat# 32010
High-Select Fe-NTA Phosphopeptide Enrichment Kit	Thermo Fisher Scientific	A32992
Pierce™ BCA Protein Assay Kit	Thermo Fisher Scientific	Cat# 23225
Trypan Blue Solution 0.4%	Thermo Fisher Scientific	Cat# 15250-061
Deposited data
Proteomics dataset	MASSIVE proteomeXchange	ftp://massive.ucsd.edu/MSV000090645/
Experimental models: Cell lines
NK-92MI	ATCC	Cat# CRL-2408, RRID:CVCL_3755
MDA-MB-231	ATCC	Cat# HTB-26, RRID:CVCL_0062
Software and algorithms
FlowJo v10.8.1	BD Bioscience https://www.flowjo.com/solutions/flowjo/downloads
MaxQuant	https://maxquant.org	version 1.6.17.0
Perseus	https://maxquant.org	Version 1.6.15.0
R	http://r-project.org	Version 4.1.3
Other
Thermomixer	Eppendorf	5355 000.011
SDS-PAGE Vertical Electrophoresis Cell	Bio-Rad	1658005
Microcentrifuge tubes, 2 mL	Eppendorf	88379
Vortex	Heidolph	541-10000-00
Centrifuge	Beckman Coulter	A46472
Power basic power supply	Bio-Rad	1645050
12% Mini-PROTEAN® TGX™ Precast Protein Gels	Bio-Rad	4561045
Razor blade
pH indicator paper	Merck	1.09565.0001
0.2 mL Skirted 96-well Robotic Plate	Thermo Scientific	AB-1300
Silicone sealing mat. For 96 well PCR plates	Nerbeplus	04-090-0000
Acclaim PepMap RSLC 75μm × 2 cm nanoViper	Thermo Fisher	PN164534
Acclaim PepMap RSLC 75μm × 15 cm nanoViper	Thermo Fisher	PN164535
Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer	Thermo Fisher	P/N 1372090
Dionex Ultimate 3000 HPLC NCS-3500RS	Thermo Fisher	5041.0010
Autosampler WPS-3000TPL RS	Thermo Fisher	Autosampler WPS-3000TPL RS
GPS200 pipette tip	Eppendorf	0030000889
Octadecyl (C18)-bonded silica	3M	2215
Tube with round bottom for culture 5 mL	Falcon	Cat# 352054
Protein LoBind tubes, 5 mL, with snap cap	Eppendorf	Cat# 0030108302
Tube, 15 mL, pp, 17/120 mm, conical bottom	Greiner	Cat# 188271
Cell culture flask, 550 mL, 175cm2, PS	Greiner	Cat# 660175
Cell culture flask, 250 mL, 75cm2, PS	Greiner	Cat# 658175
BD FACS Aria flow cytometer	Becton Dickinson Biosciences