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. 2023 Feb 8;11:1127594. doi: 10.3389/fcell.2023.1127594

FIGURE 6.

FIGURE 6

In vivo Immunomodulatory properties of the engineered EVs. (A) Representative confocal micrographs of calvarial wound beds at days 1, 3 and 7 post wounding immunostained for iNOS inflammatory macrophage marker (green) and counter stained with DAPI nuclear stain (blue) followed (below) by quantitation of average number of cells expressing iNOS per field of view. Note the significant reduction in iNOS expressing cells at days 1 and 7 in the EV treated groups and the significant reduction in 424 EV group compared to both control and control EV groups at day 1. (B) Representative confocal micrographs of calvarial wound beds at days 1, 3 and 7 post wounding immunostained for Arg1 reparative macrophage marker (green) and counter stained with DAPI nuclear stain (blue) followed (below) by quantitation of average number of cells expressing Arg1 per field of view. Note the significant increase in Arg1 expressing cells at days 1, 3 and 7 in the 424 EV treated group compared to both control and control EV groups. * in both graphs represents significance with respect to control and # represents significance with respect to control EV group measured by Tukey’s test post ANOVA.