Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 8;14:1127515. doi: 10.3389/fimmu.2023.1127515

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Kirk, Huang, Vrba, Rahman, Block, Murphy, White, Namugenyi, Ly, Tischler and Liang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Generation of rP18tri-based viral vector vaccines encoding Mtb antigens. (A) Genomic organization of the viral vectored TB vaccines (TBvac-1, TBvac-2 and TBvac-10), with the inserted Mtb antigens shown below. The P2A linker sequence was used to express two antigens from one ORF. (B) Detection of Mtb Ag85B antigen expression by Western blotting. Vero cell lysates collected 24 hours after mock infection or infection with vector, TBvac-1, or TBvac-2 were analyzed by Western blotting with anti-Ag85B and anti-GAPDH antibodies. (C) Detection of Mtb antigen expression in the infected Vero cells by IFA with anti-ESAT-6 and anti-Ag85B antibodies.