Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 8;14:1127515. doi: 10.3389/fimmu.2023.1127515

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Kirk, Huang, Vrba, Rahman, Block, Murphy, White, Namugenyi, Ly, Tischler and Liang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

rP18tri-based TB vaccines induced multifunctional CD4 T cells. (A) BL6 mice (N=5) were immunized with rP18tri vector (V), TBvac-2, and TBvac-10 through IN-IN prime-boost strategy. At 7 dpb, PBMCs were collected and incubated in medium only or with pooled Ag85B/ESAT6 antigens, and stained for cell surface markers and cytokines. Representative flow cytometry plots of CD4 T cells (CD44^hCD4⁺CD3⁺) positive for dual cytokine expression (IFNγ/IL-2, IFNγ/TNFα, and IL-2/TNFα) after either medium or peptide stimulation were shown for each immunization group (B). After normalization with the medium control, the average percentages of dual- and triple-positive (IFNγ/IL-2/TNFα) CD4 T cells after peptide stimulation were shown for each group (C). *p<0.05.