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. 2023 Feb 6;6(2):543–551. doi: 10.1021/acsabm.2c00854

Figure 5.

Figure 5

Cell migration evaluation based on calcein AM fluorescence-stained area. (A) MSC spheroids encapsulated in CNF hydrogels of different wt % concentrations migrated overtime from the initial spheroid mass into the hydrogel matrix. (B) Image analysis pipeline using CellProfiler software for calculating the fluorescence-stained area of the images. (C) Quantification of MSC migration capacity in the different hydrogel samples overtime, based on image analysis data. Significant increase of the extent of migration in all samples, relative to day 1 (a = 0.05).