Figure 2.

Mast cells promote the neurite growth and Ca2+ influx in DRG cells via GPR30. (A) Expression of Gper1 gene in mast cells, as examined by agarose gel electrophoresis (n = 3 in each group). (B) Gper1 expression in mast cells, as examined by RT-qPCR (n = 6 in each group). (C) GPR30 protein expression in mast cells, as examined by western blot (n = 3 in each group). (D) Representative images of cell morphology and neurite growth in DRG cells treated with different supernatants from mast cells for 24 h. (E) Quantified results of length neurites in different groups (n = 4, 5, 3, 3). (F) Quantified results of number of neurites in different groups (n = 6, 9, 6, 6). (G) Fluo-4 fluorescence intensity of F11 cells treated with different supernatants from mast cells. (H) Ca²⁺ peak increase (ΔF/F0) in F11 cells treated with different supernatants from mast cells (n = 3 in each group). The concentration of E₂ is 100 nM. *P < 0.05, **P < 0.01, NS, not significant.