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. 2023 Jan 10;36(2):177–187. doi: 10.1021/acs.chemrestox.2c00259

Figure 4.

Figure 4

Inhibitory effects of CBD and 7-OH-CBD in microsomes from CYP- and UGT-overexpressing HEK293 cells. (A) Incubations performed using CBD as the inhibitor; (B) incubations performed using 7-OH-CBD as the inhibitor. Shown are averages of triplicate plots comparing CBD or 7OH-CBD concentration (X-axis) with the percent of control activity (Y-axis) against substrates in CYP- or UGT-overexpressing microsomes. Incubations were performed for 30–120 min at 37 °C using 30–100 μg of microsomal protein, 0.5–120 μM of either CBD or 7-OH-CBD, and 100 μM (CYP2A6) and 500 μM (CYP2B6) nicotine for cotinine formation reactions, 50 μM (CYP2A6) and 100 μM (CYP2B6) nicotine for nornicotine formation reactions, 100 μM cotinine for 3HC formation reactions, and 30 mM 3HC for 3HC-Gluc formation reactions. Individual metabolites were analyzed by UPLC-MS/MS as described in the Experimental Procedures section.