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. 2023 Feb 8;13:1094123. doi: 10.3389/fonc.2023.1094123

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Copyright © 2023 Sisler, Hinz, Le, Kleczko, Nemenoff and Heasley

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chemokine mRNA and protein expression in MRTX-1257-treated murine KRAS^G12C cell lines. (A) LLC 46 NRAS KO, CMT-KRAS-G12C.54 and mKRC.1 cells were treated for 6 hrs to 3 days with DMSO or 30 nM MRTX-1257, total RNA was collected and submitted to RNAseq. The expression levels (in CPMs) of the indicated chemokine genes where normalized to Z-scores and graphed as a heatmap. (B) LLC 46 NRAS KO and mKRC.1 cells were plated in 6-well plates and treated for 2 days with either DMSO control, RMC-4550 (100 nM), MRTX-1257 (100 nM), or the combination. Conditioned cell media was collected and assessed for CXCL10 expression by ELISA. The data are presented as pg/μg of cellular protein and are the mean and SEM of 6 and 3 replicates for LLC 46 NRAS KO and mKRC.1, respectively. The data were submitted to 1-way ANOVA with multiple comparisons test. ns, not significant.