Fig. 3. (A) Laser scanning confocal microscopy images of seedling roots of Arabidopsis thaliana stained with APMem-1 (10.0 μM probes incubated for 5 min) at various time points. Scale bar = 20 μm (global graph). (B) Enlarged high-resolution images of selected cells of seedling roots of Arabidopsis thaliana stained with APMem-1. Scale bar = 10 μm (enlarged view). (C) The average value of fSignal/fBackground for APMem-1-stained cells versus imaging time from 0.5 h to 10 h. (D) Statistical analysis of the imaging integrity of selected cells using APMem-1 versus imaging time from 0.5 h to 10 h. (E) The value of SImaging from high-resolution pixel analysis versus imaging time from 0.5 h to 10 h. SImaging and SBackground represent the ratios of signal area and background area to the whole area.
