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. 2023 Feb 1;11:1103719. doi: 10.3389/fcell.2023.1103719

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Copyright © 2023 Tremmel, Mikat, Gupta, Mitchell, Curran, Menadue, Odorico and Sackett.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Resources used in this study. (A) Schematic diagrams for two SC-islet differentiation protocols, Protocol A (“A”) and Protocol B (“B”). Full details of these protocols are detailed in Supplemental Tables 1-3. (B) Adult human pancreas tissue (age range 24–58 years old), isolated adult human islets (“AHI”), human fetal pancreas (“HFP”) (age range 17–20 gestational weeks) and matured HFP grafts (32 weeks post-transplant) are used in this study for assessment of gene expression, protein localization, and function.