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. 2023 Feb 1;11:1103719. doi: 10.3389/fcell.2023.1103719

FIGURE 2.

FIGURE 2

UCN3 expression in SC-islets does not correlate with improved function. (A) Immunostaining for insulin (green), glucagon (blue) and UCN3 (red) in adult human pancreas (A), and end-stage cells collected after differentiation using Protocols A (B) and B (C). Images of individual channels are the same magnification as the merged images. Scale bars are 50 microns. (B) Quantification of co-localization of UCN3 with insulin in adult human pancreas, Pro A or Pro (B). (C) Quantification of co-localization of UCN3 with glucagon in adult human pancreas, Pro A or Pro (B). (D) Gene expression of UCN3 normalized to beta-actin, in adult human pancreas and SC-islets derived via Pro A or Pro B relative to undifferentiated cells (H1). (E) Static GSIS assay to assess insulin secretion in response to low glucose (LG, 2.8 mM), high glucose (HG, 28 mM) and KCl (30 mM) measured by the concentration of human C-Pep in the supernatant (pmol C-Pep/IEQ). (F) Glucose-mediated stimulation index (SI) (C-pep secreted under high glucose/C-pep secreted under low glucose) calculated from the static GSIS assay. (G) Potassium-mediated SI (C-pep secreted under KCl/C-pep secreted under low glucose) calculated from the static GSIS assay. Statistical indicators directly above each bar are compared to adult pancreas or AHI (ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).