FIGURE 5.

Glycolytic pathway is enriched in short RNA binding proteins. (A) Analysis of proteins found in snRIC_2C experiment. (B) Go-term molecular function and Interpro domain analysis of the proteins enriched in the CL short fraction in snRIC_2C experiment. (C) Schematic representation of glycolysis and TCA cycle pathways. Proteins highlighted in green were identified as long or wide range RBPs, and proteins highlighted in red were found to bind short RNAs in snRIC_2C. (D) Analysis of KEGG enriched pathways after snRIC_2C. Aminoacyl-tRNA biosynthesis and carbon metabolism pathways were found to be statistically enriched from the proteins detected in the short RNA fraction in snRIC_2C experiment. (E) Validation of glycolytic and TCA enzymes as short RBPs by 2C-WB. TAP-tagged strains and an untagged WT strain were UV-cross-linked and 1 mg of lysate was used in a round of total 2C-RNA extraction. Blots were probed against PAP antibody. The untagged WT strain was tested as a negative control for the western blot. Pab1-TAP and Pfk2-TAP were used as controls as wide range and long RNA binders, respectively. Tdh3-TAP, Fba1-TAP, Pgk1-TAP, Hxk2-TAP, Gpm1-TAP, Tpi1-TAP, and Eno1-TAP were tested as short RNA binders.