FIG 2.

TDE0201 resides in a gene cluster that is regulated by a σ⁷⁰ promoter. (A) Diagram showing the genes adjacent to TDE0201. Arrows represent the relative positions and orientations of RT-PCR primers that span the intergenic regions between individual genes as labeled. (B) RT-PCR analysis. For each pair of primers, chromosomal DNA was used as a positive control. The numbers below the primers are predicted sizes of RT-PCR and PCR products. (C) 5′-RACE analysis. The arrow shows the sequencing direction, and an asterisk indicates the transcriptional start site (TSS). The red underlined sequence (GTG) is the start codon of TDE0200. (D) Sequence comparison between the canonical E. coli σ⁷⁰ promoter sequence and that identified upstream of TDE0200.