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. 2023 Feb 8;14:1113442. doi: 10.3389/fmicb.2023.1113442

Figure 1.

Figure 1

Retapamulin-assisted ribosome profiling (Ribo-RET) profile of S. typhimurium T3E SseL pointing to translation initiation at a newly identified upstream TIS. Bacterial ribosomes are specifically arrested at translation start sites by the antibiotic retapamulin. Here, translation initiation at a newly identified upstream TIS resulted in translation of an N-terminal extended SseL proteoform (Chromosome: 2392442–2393461). The annotated and newly discovered TISs are marked with a blue and orange arrowhead, respectively. Data from Fijalkowski et al. (2022).