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(A) Protein profiles of uninduced (UN) and induced (IN) E. coli cells containing pQE80L-AMY and pQE80L-PULL (top left) and pET41a-CO-AMY (top right) recombinant plasmids. (B) Purified 6×His-tagged amylase (Amy) (bottom left) and pullulanase (Pull) (bottom middle) and GST-6×His-tagged α-amylase (bottom right). M, molecular weight markers.
