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Positive overlap between metagenomic next-generation sequencing (mNGS) and culture cultures in spinal infected lesions, pathogen species and frequencies detected by both methods are plotted in histogram (A). (B) Pie chart shows the classification of mNGS detection results for 92 spinal infection patient samples. mNGS, metagenomic next-generation sequencing; MTBC, Mycobacterium Tuberculosis Complex; NTM, nontuberculous mycobacteria.
