Fig. 2.

Overexpression of circFBXO7 inhibited NSCLC cell proliferation in vitro and in vivo. (A) The efficiency of transfected circFBXO7 overexpression vector (circFBXO7-OE) in A549 and H226 cells was detected by qRT-PCR. (B,C,D,E,F) The effect of circFBXO7-OE on A549 and H226 cell proliferation was detected by CCK-8 assays (B), colony formation assays (C, D), and EdU assays (E, F). Blue, Hoechst-stained nuclei; red, EdU-positive nuclei; scale bars =100 μm. (G, H) The percentages of cells in the G1, S, or G2 phases in A549 and H226 cells were detected by flow cytometry. CircFBXO7 arrested the A549 and H226 cell cycle at the G1/S phase. (I, J) Tumor weights and volumes of resected tumors were measured 4 weeks after inoculation of circFBXO7-transfected H226 cells (n = 3). ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NSCLC, non-small cell lung cancer. Vector-NC, negative control vector.