Fig. 4.

KLF15 inhibited NSCLC cell proliferation by inducing a cell cycle arrest at the G1/S phase. (A) The efficiency of transfected KLF15 overexpression vector (KLF15-OE) in A549 and H226 cells was detected by western blotting assay. (B,C,D,E,F) The effect of KLF15-OE on A549 and H226 cell proliferation was detected by CCK-8 assays (B), colony formation assays (C, D), and EdU assays (E, F). Blue, Hoechst-stained nuclei; red, EdU-positive nuclei; scale bars =100 μm. (G, H) The percentages of cells in the G1, S, or G2 phases in A549 and H226 cells were detected by flow cytometry. KLF15 arrested the A549 and H226 cell cycle at the G1/S phase. (I, J) Tumor weights and volumes of resected tumors were measured 4 weeks after inoculation of H226 cells transfected with negative control vector (vector-NC), miR-296-3p mimics, KLF15-OE, and miR-296-3p mimics plus KLF15-OE (n = 7). ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. NSCLC, non-small cell lung cancer.