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. 2023 Jan 23;120(5):e2210361120. doi: 10.1073/pnas.2210361120

Fig. 1.

Fig. 1.

Depletion of PCIF1 in human cells restricts coronavirus infection. A, Western blot analysis of Calu-3 cells subjected to CRISPR/Cas9 editing with PCIF1-targeting or non-targeting sgRNAs. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as a loading control. B and C, qRT-PCR analysis of viral nucleocapsid mRNA in PCIF1-KO and control Calu-3 cells or culture supernatants after infection with authentic SARS-CoV-2 (B) or Omicron (C) at an MOI of 0.1 for 1 h. Data are expressed as the mean ± SD (n = 3). ***P < 0.001 by Student’s t test. D–J, Luciferase assays quantifying infection of PCIF1-KO and control Calu-3 cells with luciferase-expressing SARS-CoV-2 (D), SARS-CoV (E), MERS-CoV (F), VSV (G), Delta (H), Beta (I), and Omicron (J) pseudoviruses. Data are presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test.