Fig. 2.

844 to 848 mutants cause wild-type neurofibromin to be destabilized. (A) Lysates from 293T NF1^−/− where HA-neurofibromin WT was cotransfected with increasing levels of either WT or mutant (FLAG-tagged). (B) Quantification of A with linear regression trendline. (C) Lysates collected from 293T NF1^−/−transfected of WT or mutant neurofibromin and treated with 100 μg/mL cycloheximide for the indicated times. Band intensity was quantified (error bars represent SD from 3 independent experiments).