FIGURE 4.

The effect of Class IIa HDACs and HDAC6 inhibition on LPS-induced TER increase of HLMVEC monolayer. HLMVEC were plated in ECIS arrays (8W10E). Baseline was set, then cells were challenged with either vehicle (0.1% dimethyl sulfoxide) or (a) TMP269 (100 nM) or (b) Tubastatin A (5 μM) for 2 h, then LPS (0.2 μg/ml) was added and TER was monitored for 12 h. Results are expressed as mean ± SEM (n = 4 experiments). ECIS, electric cell impedance sensing system; HDACs, histone deacetylases; HLMVEC, human lung microvascular endothelial cell; LPS, lipopolysaccharide. ***^,###p < .0001