FIGURE 6.

Inhibition of HDAC6, but not Class IIa HDACs increased tubulin acetylation and restored microtubule structure after LPS treatment. HLMVEC were plated in 12 well plates on glass coverslips until grown to confluence. Cells were pretreated either with Class IIa HDAC inhibitor (TMP269, 100 nM), HDAC6 inhibitor (Tubastatin A, 5 μM) or vehicle (control, 0.1% dimethyl sulfoxide) for 2 h then challenged with LPS (0.2 mg/ml) for 4 h and immunofluorescent staining was performed to visualize the acetylated tubulin. n = 3. Scale bar = 100 μm. HDACs, histone deacetylases; HLMVEC, human lung microvascular endothelial cell; LPS, lipopolysaccharide