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. 2022 Dec 29;21(3):621–634. doi: 10.1111/pbi.13976

Figure 7.

Figure 7

GFD1 could interact with OsSUT2 and OsSWEET4. (a) Yeast two‐hybrid assays (Y2H) showed that MATE1 could interact with OsSUT2 and OsSWEET4. Serial dilutions (1, 1/10, 1/100) of yeast transformant were plated onto the medium of QDO/X‐α‐gal for further screening. Co‐transformation of pGADT7‐T (T) and pGBKT7‐53 (53) was used as the positive control. The transformant of pGADT7‐T (T) and pGBKT7‐lam (lam), pGADT7 (AD) and pGBKT7 (BD) were used as the two negative controls. DDO, SD/‐Leu/‐Trp; QDO, SD/‐Leu/‐Trp/‐Ade/‐His. (b) LCI assay of GFD1 with OsSUT2 (up) or OsSWEET4 (down) in tobacco leaves. Coloured scale bars indicate the luminescence intensity in counts per second (cps). (c) Bimolecular fluorescence complementation assay analysis. nYFP‐GFD1 with cYFP‐OsSWEET4, cYFP‐GFD1 with nYFP‐OsSWEET4 were co‐transformed into tobacco leaves. nYFP, N‐terminal YFP; cYFP, C‐terminal YFP. nYFP‐GFD1 with cYFP, cYFP‐GFD1 with nYFP served as negative controls. Scale bars, 20 μm.