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. 2023 Jan 4;21(3):635–645. doi: 10.1111/pbi.13977

Figure 2.

Figure 2

Transient expression of the PDLP5 mutant 3C3A accelerates foci development of TMV‐GFP in N. benthamiana. (a) A schematic diagram illustrating the experimental set‐up and representative photographs of leaves showing the effect of PDLP5 and 3C3A on TMV foci development. Agrobacterial strains used in pre‐treatment were diluted to OD600nm = 0.4 and that for TMV‐GFP to OD600nm = 0.02. (b) Representative fluorescent images showing the relative development of TMV‐GFP foci in leaves expressing PDLP5 or 3C3A or a 1:1 mixture of these. The final OD600nm of each agrobacterial strain is 0.5. Intact leaves were photographed without detaching from the plants. Mock, empty vector. Images were taken under a BlackRay UV lamp using a D3500 Nikon camera.