Figure 2.
Biochemical characterization of p11D7. Protein A‐purified, p11D7 and an IgG isotype control were subjected to SDS‐PAGE under reducing (a, Lanes 1 and 2) or non‐reducing conditions (a, Lanes 3 and 4) and total protein content was stained with Coomassie blue. In parallel, SDS‐PAGE‐separated proteins under non‐reducing (b) or reducing conditions (c and d) were transferred to PVDF and probed for human kappa light (b and c) or for human gamma chain (d). Lanes 1 and 3: IgG isotype control. Lanes 2 and 4: p11D7. HC: heavy chain. LC: light chain. (HL)2: assembled heterotetrameric form of IgG. One representative result of multiple experiments is shown.