Table 1.
Glycan analysis of m11D7 and p11D7
| Major N‐glycan species | Schematic presentation | m11D7 (%) | p11D7 (%) |
|---|---|---|---|
| GnGn/MGn |
|
5.7 | 91.1 |
| AGn/AA |
|
12.3 | |
| GnGnF6/MGnF6 |
|
25.7 | |
| AMF6 |
|
3.3 | |
| AGnF6 |
|
33.4 | |
| AAF6 |
|
19.5 | |
| Man7‐9 |
|
0.1 | 8.9 |
Heavy chains of either hybridoma‐made 11D7 (m11D7) or 11D7 produced in ΔXFT Nicotiana benthamiana plants (p11D7) were extracted after SDS‐PAGE, trypsin digested and analysed by LC‐ESI‐MS. Glycopeptide peaks were identified using FreeStyle 1.8 and assigned percentages based on approximate molar ratios from the peak heights. Consortium for Functional Glycomics nomenclature was used.
Mannose; 
N‐acetylglucosamine; 
Fucose; 
Galactose.