TABLE 3.

Molecular activity scores in 35 DSA-positive vs 45 DSA-negative MMDx AMR/mixed biopsies

Transcript sets and classifiers		MMDx no rejection(N = 164)	MMDx mixed and AMR (N = 80)
			DSA-negative(N = 45)	DSA-positivea(N = 35 )	DSA-positive vs DSA-negative AMR/mixed(Wilcoxon test P)
AMR-related	AMR (AMRProb) classifier	0.06	0.53	0.64	0.05
	Glomerular double contours (cg > 0Prob) classifier	0.13	0.44	0.57	0.05
	Glomerulitis (g > 0Prob) classifier	0.14	0.58	0.66	0.06
	Peritubular capillaritis (ptc > 0Prob) classifier	0.13	0.66	0.78	0.004
	DSA probability (DSAProb) classifier	0.29	0.60	0.68	0.03
All rejection	Rejection (RejProb) classifier	0.07	0.66	0.80	0.005
TCMR-related	TCMR (TCMRProb) classifier	0.02	0.09	0.15	0.37
	Interstitial infiltrate (i > 1Prob) classifier	0.05	0.24	0.27	0.38
	Tubulitis (t > 1Prob) classifier	0.05	0.17	0.24	0.60
Macrophage-related	Constitutive macrophage-associated transcripts (QCMAT)	0.32	0.74	0.76	0.67
	Alternative macrophage activation transcripts 1 (AMAT1)	0.43	0.97	1.07	0.16
Recent injury	Injury/repair associated transcripts (human kidney) (IRRAT30)	0.32	0.68	0.60	0.67
	Injury-repair induced transcripts, d 3 (IRITD3)	0.04	0.16	0.13	0.95
Normal parenchymal	Kidney transcripts – set 1 (KT1)	–0.28	–0.47	–0.48	0.67
Atrophy fibrosis	Interstitial fibrosis (ci > 1Prob)	0.35	0.53	0.61	0.18
	Tubular atrophy (ct > 1Prob)	0.29	0.45	0.52	0.31

Bold indicates P < 0.05; bold and underline indicates P < 0.01.

Shading indicates rows with AMR-related variables.

^aIncludes PRAHR biopsies. Biopsies from PRA-positive patients with missing/unavailable donor phenotyping to assign DSA status were called PRAHR in this study and were analyzed as DSA-positive.

AMR, antibody-mediated rejection; DSA, donor-specific antibody; MMDx, Molecular Microscope Diagnostic System; PRA, panel-reactive antibody; PRAHR, panel-reactive antibody–high risk, TCMR, T cell–mediated rejection.