Figure 1.

A. Synthesis and Characterization of mPEG-CS-cRGD/Bmi-1RNAi-PTX. ¹H-NMR of CS (a), mPEG-COOH (b) and mPEG-CS (c). B. TEM showed that the nanocomposite was approximately spherical with a particle size of about 200 nm (a: 50 nm, b: 200 nm). C. The mPEG-CS carrier has the ability to encapsulate DNA and resisted nuclease degradation. a When N/P ratio was 8, pDNA was completely wrapped. b When N/P ratio was less than 5, the DNA bands disappeared. D Package with DNA ability and anti-nucleic enzyme degradation ability of mPEG-CS. a mPEG-CS-cRGD NPs have the better transfection effect to naked DNA. b mPEG-CS-cRGD/Bmi-1RNAi-PTX NPs silenced Bmi-1 gene expression. We use one-way ANOVA exposure, control and pDNA vs. other two groups, f(3)=36.786, p < 0.001.