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. 2023 Feb 20;12(1):2178238. doi: 10.1080/22221751.2023.2178238

Figure 1.

Figure 1.

NSUN2 negatively regulates antiviral innate type I interferon responses. (a) qPCR analysis of IFNB1 mRNA in HEK293T cells transfected with siControl or siRNAs targeting different RNA methyltransferases or demethylases for 36 h, with or without infection by SeV for another 8 h. (b) Dual-luciferase assay analysing IFN-β promoter activity (IFN-β-Luc) in HEK293T cells in 24-well plates transfected for 24 h with 100 ng IFN-β-Luc plasmid and 20 ng Renilla luciferase plasmid (RL-TK) along with vector or increasing amounts (0, 0.1, 0.2, and 0.5 μg) of plasmid encoding NSUN2, with or without infection by SeV, for another 10 h. (c) Dual-luciferase analysis of IFN-β-Luc in HEK293T cells in 24-well plates transfected for 24 h with vector (Vec) or NSUN2, with or without infection by SeV or VSV for another 10 h, or transfected with poly (I:C) (1 µg/mL) for another 10 h. (d) qPCR analysis of IFNB1, ISG15, CXCL10 and NSUN2 mRNA in HEK293T cells transfected with siControl or siRNAs targeting NSUN2, with or without infection by SeV for 8 h. Immunoblot analysis shows knockdown efficiency of siRNAs targeting NSUN2. (e) qPCR analysis of IFNB1, ISG15 and CXCL10 mRNA in wild-type HEK293T cells or NSUN2−/− HEK293T cells, with or without infection by SeV for 8 h.