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. 2023 Feb 20;12(1):2178238. doi: 10.1080/22221751.2023.2178238

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Knockout of NSUN2 decreased m⁵C methylation on IRF3 mRNA but increased IRF3 and IFNB1 and downstream ISGs mRNA levels. (a) RNA-seq analysis from SeV-infected A549 wild-type cells or A549 NSUN2 knockout cells. GO-term functional enrichment by biological process of up-regulated genes. Data show the top 10 biological processes. (b) The heatmap shows the expression levels of ISGs and several signalling molecules. The genes that we focus on are labelled with asterisks. (c) The column diagram shows the SeV replication levels. (d) The m⁵C-RIP-qPCR analysis of the m⁵C methylated mRNA levels immunoprecipitated by m⁵C antibody from A549 wild-type cells or A549 NSUN2 knockout cells. Data are representative of three independent experiments and were analysed by two-tailed unpaired t test. Graphs show the mean ± SD (n = 3) derived from three independent experiments. NS, not significant for P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.