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. 2023 Feb 20;12(1):2178238. doi: 10.1080/22221751.2023.2178238

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Pivotal role for NSUN2 in the induction of type I interferon and antiviral response in vivo. (a) Immunoblot analysis in BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice with infection by SeV for 0, 4, and 8 h. (b) qPCR analysis of Nsun2 or Irf3 mRNA in BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice. (c) qPCR analysis of Ifnb1 mRNA in BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice, with or without infection by SeV for 8 and 12 h, HSV-1 for 3 and 6 h, or VSV for 6 and 12 h. (d) qPCR analysis of Isg15 and Cxcl10 in BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice, with or without infection by SeV, HSV-1, or VSV. (e) qPCR analysis of SeV-P, VSV-G or HSV-1-UL-30 RNA in BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice, with infection by SeV, VSV or HSV-1 for 12 h. (f) RNA-seq analysis from SeV-infected BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice. The heatmap shows the expression levels of ISGs and several signalling molecules. The genes that we focus on are labelled with asterisks. (g) The m⁵C-RIP-qPCR analysis of the m⁵C methylated mRNA levels immunoprecipitated by m⁵C antibody from BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice. (h) Stability analysis of Irf3 mRNA in BMDCs from Nsun2^+/+ mice or Nsun2^+/− mice, with treatment of actinomycin D (ActD) for 0, 4, and 8 h. (i) qPCR analysis of Ifnb1 mRNA and the corresponding VSV-G RNA in different organs from Nsun2^+/+ mice (n = 5) or Nsun2^+/− mice (n = 5), injected intraperitoneally for 16 h with VSV (4 × 10⁷ PFU per mouse).