Fig. 4. Validation of two giant proteins, GSBP1 and GSBP2.

(A) The validation of GSBP1 gene model; (B) The validation of GSBP2 gene model. The gene model was predicted as described in Materials and Methods. No introns were found in either GSBP1 or GSBP2. Nanopore reads: The raw sequencing reads mapping back to genome assembly. Note that only reads that span the whole gene body are shown. RNA-seq: Read coverage of transcriptome sequencing of total RNA without poly(A) mRNA purification. R1 and R2, replicates 1 and 2. MS: The peptides matched to GSBP1 and GSBP2 in whole-cell protein MS identification. Note that GSBP1 and GSBP2 have many internal repeats, and if a peptide matched multiple loci, then all of these are shown.