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. 2023 Feb 22;9(8):eadd6550. doi: 10.1126/sciadv.add6550

Fig. 5. Colocalization of spasmin and GSBP in S. minus.

Fig. 5.

(A) AlphaFold2 prediction of partial GSBP sequences (green) and spasmin (cyan). (B) Colocalization of GSBP2 and spasmin. Red: IF using anti-centrin antibody (mouse) represents spasmins that have high abundance in the cell based on MS result. Green: IF using anti-GSBP2 antibody (rabbit). Blue and yellow arrowheads: Contractile fibrillar bundles running in different directions. Note that all layers of IF images are merged and shown. (C) Localization of GSBP1 and GSBP2. Red: IF using anti-GSBP1 antibody (mouse). Inset: Western blot result of GSBP1. Green: IF using anti-GSBP2 antibody (rabbit). Inset: Western blot result of GSBP2; the two bands are GSBP1 and GSBP2, respectively. Note that only one layer of IF images is shown.