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. 2023 Feb 6;9(2):238–254. doi: 10.1038/s41477-022-01333-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Recombination rate per chromosome, finding chr. 20 has a much lower rate of recombination than expected from the other 19 chromosomes. b, Sliding window analyses (100,000 bp window, 10,000 bp jump) of nucleotide diversity and F_ST between S. divinum chr. 20 SNP clusters. c, Exact k-mer dotplot with word size 15 for the shared sequence region between chr. 20 (putative V) and Scaffold9707 (putative U fragment), assembled from suspected female genotypes. d, S. angustifolium competitive mapping assay between chr. 20 and Scaffold9707. Ratio of reads mapped to the shared U/V region are shown, with individuals mapping to one sequence or the other (NA-ambiguous mapping ratio). Null distribution of autosome pairwise ratios is shown in yellow. e, Sphagnum diversity panel competitive mapping assay. Regardless of subgenera, individuals either mapped preferentially to the shared region of chr. 20 or Scaffold9707. Monoicous species (S. squarrosum, S. compactum, S. strictum and S. fimbriatum) each preferentially mapped to chr. 20. Positions on plot have been randomly ‘jittered’ by 0.1 units to improve readability among points.

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