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. 2023 Feb 6;9(2):238–254. doi: 10.1038/s41477-022-01333-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a) Violin plot depicting gene-wide rate ratios of non-synonymous (dN) to synonymous (dS) substitution for genes on autosomes (n = 1,425) and chr.20 (n = 30) across the Sphagnum diversity panel. Higher values of dN/dS in genes on chr.20 suggest relaxation in the strength of purifying selection, positive selection, or a combination of both positive and relaxed purifying selection as compared to autosomal genes. Dots and bars represent mean values ± one standard deviation, respectively. b) S. angustifolium pedigree competitive mapping assay. Reads from each pedigree library were mapped to the genome assembly of S.angustifolium, along with the scaffold sequence Scaffold9707. Reads mapped to chr. 20 and Scaffold9707 are expressed as the total percentage per reads within the fastq file. c and d) Polymerase chain reaction results of male-specific (panel c) and female specific (panel d) primers used for amplification with Sphagnum samples of known sex (metadata details provided in Supplementary Table 12), Expected amplicon size for the PCR reaction is 444 bp (panel c) and 394 bp (panel d). PCR amplicons were separated on a 2% agarose gel, run for 2 hours at 80 volts. GeneRuler DNA ladder was run in lanes 1 and 20. DNA from female Sphagnum samples were loaded into lanes 2–19;21–22 and DNA from males was loaded into lanes 23–34. Individual gel results were not replicated but were independently consistent (for example male samples were 100% positive for male specific primers (panel c) and 100% negative for female-specific primers (panel d).

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