Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 15;614(7949):732–741. doi: 10.1038/s41586-023-05711-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article′s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article′s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a, Scheme of the experimental design. Nuclei were isolated from hippocampi of Npas4^fl/fl and Tip60^fl/fl mice expressing either Cre-mCherry or ΔCre-GFP, followed by snRNA-seq. An additional PCR step to amplify viral transcripts within the cDNA library was used to assign infection status to each nucleus. b, Left, uniform manifold approximation and projection (UMAP) visualizations of Npas4^fl/fl and Tip60^fl/fl snRNA-seq datasets. Right, UMAP visualizations of infection status. Npas4^fl/fl: 32,418 nuclei from 2 mice, 12,963 Cre-infected, 8,845 ΔCre-infected. Tip60^fl/fl: 44,511 nuclei from 3 mice, 13,536 Cre-infected, 13,461 ΔCre-infected. c, Boxplots showing the average expression (Seurat log(e) normalized counts) of NPAS4 or TIP60 target genes (Methods) comparing Cre-infected and ΔCre-infected nuclei within each neuronal celltype. Boxplots show the median (line), inter-quartile range (IQR; box) and 1.5× IQR (whiskers), and notches indicate the median ± 1.58× IQR/sqrt(n). ***P < 5 × 10⁻¹⁰, **P < 5 × 10⁻⁴, *P < 0.01. P values were calculated using unpaired, two-tailed Wilcoxon rank-sum tests. Exact P values and cell numbers per cluster provided in the source data. d, Schematic of the recording configuration, showing the stimulating electrode in the centre of the stratum pyramidale to measure IPSCs onto neighbouring Cre-infected and uninfected CA1 pyramidal neurons in either Npas4^fl/fl or Tip60^fl/fl acute hippocampal slices. L, lacunosum; O, oriens; P, pyramidale; R, radiatum. e, Representative image (performed in triplicate) showing sparse Cre-mCherry expression in the CA1. f,g, Scatterplots of IPSC amplitudes recorded from pairs of neighbouring uninfected and Cre-infected cells in unstimulated (Unstim.; saline) or stimulated (Stim.; low-dose KA) Npas4^fl/fl (f) or Tip60^fl/fl(g) mice. Points represent an uninfected–Cre-infected pair. Squares represent the average ± s.e.m. Right insets, traces of uninfected or Cre-infected cells. Npas4^fl/fl: stimulated: n = 16 pairs from 4 mice; unstimulated: n = 12 pairs from 5 mice. Tip60^fl/fl: stimulated: n = 11 pairs from 2 mice; unstimulated: n = 12 pairs from 2 mice. *P = 3.73 × 10⁻³ for Npas4^fl/fl; *P = 0.0209 for Tip60^fl/fl. Data are mean ± s.e.m. P values were calculated using unpaired, two-tailed t-tests. The sequencer image in a is from BioRender (https://biorender.com).

Source Data