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. 2023 Feb 15;614(7949):732–741. doi: 10.1038/s41586-023-05711-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article′s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article′s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a. Immunohistochemistry image of an Npas4^fl/fl mouse injected with AAV to express Cre-mCherry (shown in red) and collected 2 h post low-dose KA to induce NPAS4 (shown in cyan). Representative image from 3 animals. Scale bar = 1 mm. b. Immunohistochemistry image of a Tip60^fl/fl mouse injected with AAV to express Cre-mCherry (shown in red). TIP60 (shown in cyan). Representative image from 3 animals. Scale bar = 1 mm. c. Immunohistochemistry image of both hippocampal hemispheres of an Npas4^fl/fl mouse injected with Cre-mCherry and ΔCre-GFP in contralateral sides of the hippocampus and collected 2 h post low-dose KA to induce NPAS4 (shown in white). Representative image from 3 animals. Scale bar = 1 mm. d. Western blot from whole hippocampal tissue of Tip60^fl/fl mice injected with AAV expressing Cre-mCherry and ΔCre-GFP in contralateral sides of the hippocampus. Tissue was collected at either 0 or 2 h post KA stimulation. Cre and ΔCre tissue was collected from each individual mouse (0 h, n = 2; 2 h, n = 2). See gel source data (Supplementary Fig. 1). e. Quantification of the Western blot is shown in d, normalizing the NPAS4 signal to loading control GAPDH. f. Left: UMAP visualization of full Npas4^fl/fl snRNA-seq dataset. Nuclei are colored according to mouse of origin. 32,418 nuclei from 2 mice. Right: UMAP visualization of full Tip60^fl/fl snRNA-seq dataset. Nuclei are colored according to mouse of origin. 44,511 nuclei from 3 mice. g. Summary of final nuclei numbers in Npas4^fl/fl and Tip60^fl/fl snRNA-seq datasets, and quantification of infection rates with Cre-mCherry and ΔCre-GFP viruses. Higher infection rate in neurons reflects the known tropism of the AAV2/9 virus used in these experiments. h. Cell-type assignment in Npas4^fl/fl (left) and Tip60^fl/fl (right) snRNA-seq datasets using indicated marker genes. The y-axis denotes normalized expression (Seurat log_e normalized counts). i. Distribution of number of genes detected per nucleus, by cell-type. Npas4^fl/fl (left) and Tip60^fl/fl (right).

Source Data