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AKS-452 immunogenicity kinetics and IgG Isotype titers. Serum samples were obtained at Days, 0, 28, 56, 90, and 180 from the initial vaccine dose from all subjects in cohort 1 (1 × 90 µg) and cohort 2 (2 × 45 µg) and assessed for anti-SP/RBD IgG isotype titers via isotype-specific ELISAs (mean µg/mL ± SEM). *, p ≤ 0.05; ***, p ≤ 0.001; ****, p ≤ 0.0001; 2-tailed t-test (GraphPad Prism).
