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. 2023 Feb 22;9:73. doi: 10.1038/s41420-023-01364-7

Fig. 5. miR579-3p targets the KLF4 mRNA 3′UTR.

Fig. 5

Wild-type and 13 bp deletion of 3′-UTR of KLF4, was amplified from the human genome and subcloned into the XhoI and NotI sites between Renilla gene and polyA of the psiCheck2 vector. Each constructed luciferase reporter plasmid or empty vector was transfected into AoSMCs at 5000 cells per well in 96-well plates. Luciferase was assayed at post 48 h transfection using the Dual-Luciferase Reporter Assay System. Luminescence was quantitated and renilla luciferase readings were normalized against the firefly luciferase activity to determine the relative luciferase activity. Data are presented as mean ± SD (n = 8 replicates). Pairwise comparison was made through Student’s t-test: ***P < 0.001; n.s. not significant. A Schematic of the luciferase reporter vector and constructs. B The miR579-3p sequence complementary to that of the KLF4 3′UTR. C Relative luciferase activity compared between miR579-3p and miR-Con.