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. 2023 Feb 9;11:1083033. doi: 10.3389/fcell.2023.1083033

FIGURE 1.

FIGURE 1

MSK1 phosphorylation in impaired in p38δ−/− macrophages. (A) Schematic representation of the TLR4 signalling pathways involved in MAPK and NFκB pathway activation. TLR4 stimulation by LPS triggers the activation of TAK1-IKK-TPL2 via MyD88. p38γ and p38δ regulate TPL2 steady-state levels, which is in a complex with ABIN-2 and p105 (3, 8). The kinases blocked by the indicated inhibitors are shown. (B,C) BMDM from WT, p38γ−/−, p38δ−/− or p38γ/δ−/− mice were stimulated with 100 ng/mL LPS for 30 min. Cell lysates were immunoblotted with the indicated antibodies. Representative immunoblots from three independent experiments in duplicate are shown. (D) BMDM from WT or p38δ−/− mice were stimulated with 250 ng/mL CpG-ODN for the times indicated. Cell lysates were immunoblotted with the indicated antibodies. Representative immunoblots from three different experiments in duplicate are shown. Molecular weights are indicated at the side of the blots.