FIGURE 6.
LIN suppresses HSCs activation by inhibiting TGF-β/Smad signalling. (A,B) LX-2 cells were transfected with control or SHP2 siRNA (20 pmol/1.0 × 105 cells). Twenty-fourhours later, transfected LX-2 cells were treated with LIN at the indicated concentrations. Fibrogenic gene expression levels in LX-2 including ACTA2, VIM, TGF-β, FN1, DES, and COL1A1 were evaluated by qPCR. SHP2 levels in siRNA-treated cells were confirmed by immunoblotting with anti-SHP2 antibody and qPCR. Experiments were performed in three biological repeats. (C) The expression of p-Smad2/3 and t-Smad 2/3 after LIN treatment (5 and 10 μM) was detected by Western-blot.