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. 2023 Jan 12;66(4):674–694. doi: 10.1007/s00125-022-05856-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — RREB1 knockout reduces cellular insulin content in mature beta cells. (a–c) RREB1 knockout (RREB1-KO) EndoC-βH1 cells were assessed for (a) INS gene expression (% of control cells [EV], normalised to housekeeping genes TBP and PPIA), (b) cellular insulin content (pg/cell) and (c) glucose-stimulated insulin secretion (pg/ml) (n=5). (d) Forskolin-mediated insulin secretion at 20 mmol/l glucose (pg/ml) (n=3). (e–g) After stimulation with forskolin, (e) glucose-stimulated insulin secretion (pg/ml), (f) the stimulation index and (g) insulin content (pg/cell) were measured (n=3). Data are presented as means±SEM. *p<0.05, **p<0.01, p***<0.001 (a, b, d, unpaired t test; c, e, two-way ANOVA followed by Sidak’s multiple comparisons test)