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. 2023 Feb 22;6:208. doi: 10.1038/s42003-023-04563-1

Fig. 3. Generation of conditional full-length human Klotho knock-in allele and targeted insertion of human Klotho in Alb-hKL and Pod-hKL mice.

Fig. 3

a Schematic representation of ROSA26 wild-type allele (top), targeted allele (middle), and conditional knock-in allele (bottom). In the latter, full-length human Klotho expression is specifically induced in hepatocytes or podocytes by using Cre–LoxP recombination system. Albumin- or podocin-cre mice were crossed with mice expressing the targeting vector, containing a lox-STOP-lox cassette between the splice acceptor sites and Klotho cDNA under the CAG promoter, that was introduced into the ROSA26 locus. b Western blotting of whole liver extracts from WT and Alb-hKL (top) and glomeruli-enriched lysates from WT and Pod-hKL mice (bottom). c Representative immunofluorescent stainings of Klotho and GFP in liver (top) and kidney (bottom) of Alb-hKL and Pod-hKL, respectively. d Serum (top) and urine (bottom) Klotho levels in WT (n = 9), Alb-hKL (n = 5), and Pod-hKL (n = 4) mice. **p < 0.01; ***p < 0.001. Scale bars: 100 μm, 30 μm (c). Statistical significance between groups was determined using one-way ANOVA followed by multiple comparisons.