Table 1.
The detailed results of knock-in efficiency in B02-, Nocodazole-, and double-treated groups.
| Type of treatment | Total clone | Total PCRs | 5′ junction PCR positive | 3′ junction PCR positive | 5'/3' junction PCR positive | KI efficiency (%) |
|---|---|---|---|---|---|---|
| Nocodazole-treated group | 85 | 79 | 52 | 63 | 51 | 64 |
| Control group | 50 | 33 | 9 | 14 | 9 | 27 |
| B02-treated group | 70 | 62 | 37 | 55 | 36 | 58 |
| Control group | 74 | 71 | 22 | 55 | 21 | 29 |
| Double-treated group | 89 | 52 | 39 | 34 | 21 | 40 |
| Control group | 50 | 42 | 16 | 42 | 16 | 38 |