Fig. 6. The Kelch13 complex affects the integrity of the micropore.

a Micropore structures on TEM and its quantifications. Serial TEM was performed to examine different types of micropore defects in the parasite lines grown in auxin for 30 h. These defects were quantified by TEM for 600 parasites from each line. The micropore defects were indicated by colors, which relate to the graphs. Scale = 200 nm. b Plasma membrane invagination close to the apex was observed on TEM analysis in IAA induced parasites for 30 h, as in a. This invagination (yellow arrowhead) was previously shown in parasites treated with 200 µm oleic acid⁶⁰. Here it shows the tKD and TIR1 grown in auxin. Scale = 500 nm. c, d Scanning microscopy observation of the corrupted micropore in parasites with IAA for 30 h. The different types of micropore were shown with number 1, 2 and 3 in brackets, the type 1 was normal, while others were corrupted. The type 3 contains hanging membranes (c). These micropores were scored and calculated as ratios in the parasites where the micropore or its defects were observed (d) (N ≥ 40 for each line). Green scale = 1 µm, white scale = 200 nm. e Area analysis of the SEM defects as shown in c. Data were shown with mean ± SD, and analyzed by one-way ANOVA with Tukey’s multiple comparison, the exact p values are provided. Three independent experiments were performed with measurements of n > 12 (defective) micropores in each independent experiment.