REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
RPMI-1640	Millipore Sigma	R0883
DMEM	Millipore Sigma	D0822
FBS	Gibco	10082147
2-Mercaptoethanol	Millipore Sigma	M6250
12-myrisate 13 acetate	Sigma Aldrich	P8139
Penicillin streptomycin	Cell services	721
Antigen Unmasking solution	Vector Lab	H-3301
Ethanol	Fisher Scientific	BP28184
Bovine serum albumin	Sigma Aldrich	A9647-100G
Hematoxylin and Eosin	Vector Lab	H-3401
Bloxall	Vector lab	SP-6000
Xyelene	Fisher Scientific	EW-88043-30
Diluent	Vector lab	SP-5035
Tween 20	Sigma Aldrich	P7949
EDTA free Protease Cocktail inhibitor	Thermo scientific	87785
Novex 4–20% Tris Glycine Gels	Invitrogen	XP04200BOX
LPS	Sigma Aldrich	L2880
TPER	Thermo scientific	78510
Pierce BCA	Thermo fisher	23225
Adenosine Triphosphate	Sigma Aldrich	A2383
CellLines
THP-1	ATCC	TIB-202
LLC	ATCC	CRL-1642
A549	ATCC	CRM-CCL-185
RAW-ASC	Invivogen	raw-asc
Antibodies
CD107b/Mac3	BD biosciences	550292
Cleaved Il-1beta	Cell signaling technology	63124
Cleaved Gasdermin D	Cell signaling technology	50928
Beta Actin	Cell signaling technology	8457
ASC	Cell signaling technology	67824
MMP 9	Sigma Aldrich	SCP0191
AIM2	Cell signaling technology	63660
Capsase-1	Thermo Fisher	MA5-32909
Horseradish peroxidase-Conjugated goat anti rabbit secondary	Bio Rad	1662408EDU
Critical Commercial Assays
RNeasy Mini kit	Qiagen Inc Valencia, CA	74106
iScript cDNA kit	Bio Rad	1708891
VECTASTAIN Elite ABC-HRP kit	Vector lab	PK6104
Vector NovaRed kit	Vector Lab	SK-4800
Mouse IL beta ELISA Kit	R and D systems	MLB00C
Oligonucleotides
AIM2	Thermo Fisher	Mm01295719
NLRP3	Thermo Fisher	Mm0084094
Beta-Actin	Thermo Fisher	Mm02619580
Software and Algorithms
Image Pro Plus	Media Cybernetics, Inc,Rockville, Maryland USA	https://www.mediacy.com/imagepro
GraphPad Prism	GraphPad	https://www.graphpad.com/
Other
Inverted microscope	Leica Microsystems, GmbH,Wetzlar, Germany	Leica DMI6000
Hamamatsu Orca Flash4 camera	Hamamatsu Photonics, Shizuoka,Japan	Leica DMI6000
iBrightTM CL750 imaging system	Life Technology	A44116
QuantStudioTM 3 Real -Time PCR systems	Applied Biosystem	A28137