Figure 3.

Integrins α_vβ₃ and α₅β₁ are upregulated in neovascularization in flat mounts of ischemic retina

At P17, C57BL/6 room air control mice (n = 10) or mice with oxygen-induced ischemic retinopathy (n = 10, OIR) were euthanized and retinal flat mounts were stained with Alexa Fluor 594-conjugated Griffonia Simplicifolia Agglutinin (GSA) lectin and immunostained for α_vβ₃ or α₅β₁. There was no α_vβ₃ detectable on GSA-labeled retinal vessels in room air control retina (A–C), but it was detectable in buds of NV on retinal flat mounts from P17 mice with OIR (D–F). Similarly, integrin α₅β₁ was not detectable on GSA-labeled retinal vessels in room air control retina (G–I), but it was detectable in NV in retinal flat mounts (J–L) from mice with OIR. The experiment was performed in duplicates, and qualitatively similar results were found in the replicates. Scale bar = 100 μm.