Figure 4.

AXT107 co-localizes with α₅ and α_v integrins at cell-cell junctions in cultured human umbilical vein endothelial cell (HUVEC) monolayers

HUVEC monolayers were treated with 25 μM AXT107 containing 25% fluorescein amidite (FAM)-labeled AXT107 (green) and then immunohistochemically stained with antibodies against integrins α₅ (A–C) or α_v (D-F) or F-actin using rhodamine-phalloidin (G–I) (red). DAPI (blue) was included as a nuclear stain in all images. Images were taken as z series using the 63× objective and 0.3 μm step sizes. Data are presented as merged volume projections at an angle from both the top (A, D, and G) and the bottom (B, E, and H) and as a maximum intensity projection (C, F, and I). N = 2; Scale bar = 20 μm.