Figure 5.

Prenatal exposure to MeHg results in cell trajectory alterations in E13.5 cortices

(A) Velocity vectors for total cell populations in both 0ppm (control) and 0.2ppm MeHg, visualized and calculated from RNA velocity using the stochastic model, projected onto UMAP visualizations.

(B) Enhanced image of velocity vectors in the RGP1 and RGP2 population for both 0 and 0.2ppm MeHg.

(C) Quantitative analysis of velocity vectors in RGP1 directed toward the RGP2 population between 45 and 180°. Quantitative analysis of velocity vectors in RGP1 directed toward the transitional cell population between 270 and 360°. Parameters used to differentiate vector direction.

(D) Pseudotime analysis for 0 and 0.2ppm MeHg.

(E and F) Phase portraits of marker genes (Pax6, Tubb3, and Tbr1) in 0ppm (E) and 0.2ppm MeHg (F), calculated from RNA velocity.

(G) Velocity vectors for transitional cell populations in both 0 and 0.2ppm MeHg treatment groups were visualized and calculated from RNA velocity using the stochastic model, projected onto UMAP visualizations.

(H) Pseudotime analysis for the 0 and 0.2ppm MeHg treatments within the transitional cell population.

(I and J) Phase portraits of marker genes (Pax6, Tubb3, and Tbr1) in the transitional cell population for 0ppm (I) and 0.2ppm MeHg (J) treatments, calculated from RNA velocity. See also Figure S5.